The quantitative composition of microorganisms distributed in the anthropogenic type soil of Eastern Georgia (near the Tbilisi Sea), the share of nitrogen-fixing microorganisms in the microbial association, the features of their distribution and the correlation of this distribution with some physiological groups of microorganisms have been studied. It has been established that the qualitative and quantitative composition of nitrogen-fixing microorganisms changes depending on the soil profile (depth). Microbiological analysis over the five years of the study period showed that the anthropogenic type soil of Eastern Georgia is abundantly represented by nitrogen-fixing microorganisms of the genus Beijerinckia; at the same time, there is a decrease in the number of nitrifiers in the soil and an increase in the number of anaerobic bacteria.