Honey, a natural product derived from floral nectar and processed by bees, has long been recognized for its therapeutic properties. In recent years, extensive research has focused on exploring the antibacterial, anti-biofilm, and anti-quorum sensing activities of honey. This comprehensive review aims to summarize and analyze the current state of knowledge regarding the diverse antimicrobial properties of honey, with a particular emphasis on its effectiveness against bacterial infections, biofilm formation, and quorum sensing mechanisms. The review discusses the chemical composition of honey, including its various bioactive components, and examines their contributions to its antimicrobial effects. Furthermore, the mechanisms underlying honey’s antibacterial action, its ability to disrupt biofilm formation, and its interference with quorum sensing signaling pathways are elucidated. The review also highlights the potential applications of honey in clinical settings, wound management, and the development of novel therapeutic agents. By providing a thorough examination of the antibacterial, anti-biofilm, and anti-quorum sensing properties of honey, this review aims to contribute to a deeper understanding of its therapeutic potential and to inspire further research in this promising area.