Ananas comosus (Bromeliaceae) is a purely tropical perennial herb whose fruits are appreciated. The cultivated species is a self-incompatible diploid and produces few seeds. The aim of the present study is to investigate the in vitro culture conditions favorable to the rapid and mass regeneration of Ananas comosus plants in order to establish a protocol for propagation. The explant used is the apices from the crown. After disinfection, the apices taken from the crowns of Ananas comosus var. cayenne are cultured on a complete Murashige and Skoog (MS) medium containing different concentrations of Benzylaminopurine (BA), Kinetin (KIN), 2,4-Dichlorophenoxyacetic acid (2,4-D), Naphtaelene acid (NA), as well as combinations of BA/NAA and BA/2,4-D were studied. NAA promotes callogenesis with maxima of 87.5% with 3 or 4 mg.l-1 versus 100% in the presence of 1.5 or 2 mg.l-1 2,4-D. BA at 2 mg.l-1 and KIN at 3 mg.l-1 allowed the proliferation of more than 100 buds per cal. The BA/NAA ratio at 2/2 mg.l-1 allowed rooting and neoformation of leaves of vitroplants with a mean maximum number of 11.16 ± 1.4 leaves and 8.8 ± 1.2 roots per vitroplant and 11.41 ± 2.00 leaves and 9.8 ± 2.5 roots in the presence of 2/3 mg.l-1 BA/2,4-D. Some seedlings were acclimatized on a vermiculite soil mixture. Acclimatization of the vitroplants for 70 days was 86% successful. Apices are good starting materials for the indirect regeneration of Ananas comosus. Their use allows the regeneration of a large number of vitroplants and thus increases the potential for the in vitro production of healthy plant material in A. comosus.