The present investigation has been performed to evaluate Benisuif-5 durum wheat genotype for tolerance to osmotic stress at two-factor experiment in a completely randomized design. The first factor (A) included four levels of PEG6000: 0 – distilled water; 5%; 10% and 20%. The second factor (B) included four levels of NaCl: 0 – distilled water; 50 mM; 100 mM and 200 mM. Seedling length, root numbers, seedling fresh weight, seedling fresh weight and dry weight, tissue water content, tolerance index, seedling length reduction, root number reduction, seedling fresh weight reduction and seedling dry weight reduction were measured in the study under each factor. After the analysis of the obtained results, it was concluded that, all the parameters values were reduced by both stresses under the different mechanisms.