Fusarium wilt, incited by Fusarium oxysporum, limits okra productivity. Eco-friendly control strategies involving botanicals and microbial agents offer reliable mitigation options. This study investigated the in vitro effect of Trichoderma harzianum and Neem extract on F. oxysporum and evaluated the effect of T. harzianum, Glomus hoi, and Neem extract on the growth and disease severity (DS) of Okra infected with Fusarium oxysporum. Aqueous extract of Neem leaf at varying concentrations was incorporated into molten PDA and allowed to set for in vitro experiments, while 5 ml of 105 spores/ml T. harzianum and F. oxysporum, 30 ml of neem extract (0.5%), and 30 g soil inoculum of G. hoi were applied to the soil surrounding the roots of okra plants in different treatment combinations in vivo. The least radial mycelia growth (RMG) of 1.6 cm was recorded when F. oxysporum was introduced 72 hours after T. harzianum in vitro. The highest RMG of F. oxysporum (3.2 cm) was observed in plates containing 0.1% Neem extract, while the least RMG was observed on plates containing 0.5% Neem extract. Application of T. harzianum at planting and at two- and four-weeks before F. oxysporum inoculation reduced the DS, while G. hoi application 4-weeks before F. oxysporum also reduced DS. Neem extract and T. harzianum effectively reduced DS of F. oxysporum in okra. Both treatments inhibited fungal growth in vitro, while G. hoi, neem extract, and T. harzianum lowered DS in vivo. Single and combined application of G. hoi and T. harzianum improved okra growth.